Physical Biochemistry - Publications

Book Chapters

Kaas Q, Westermann JC, Henriques ST and Craik DJ (2010) Antimicrobial Peptides in Plants. In “Antimicrobial peptides, Discovery, Design and Novel Therapeutic Strategies” (Wang G, Ed.) CABI (Oxfordshire, UK), pp 40-70. ISBN: 978-1-84593-657-0.

Henriques ST (2010) Biophysics and the way out of the endosomal versus non-endosomal dilemma for cell-penetrating peptides pep-1. In "Membrane Active Peptides: Methods and Results on Structure and Function" (Castanho M, Ed.) IUL Publishers (La Jolla, CA, USA), pp 377-414. ISBN: 978-0-9720774-5-3.

Veiga AS, Franquelim HG, HIV-1 Fusion Inhibitor Peptides: A Cross Correlation between Efficacy and Membrane-Level Activity, In "Membrane Active Peptides: Methods and Results on Structure and Function" (Castanho M, Ed.) IUL Publishers (La Jolla, CA, USA), pp 495-528. ISBN: 978-0-9720774-5-3.

Short List of Selected Publications

"Arginine-rich self-assembling peptides as potent antibacterial gels." Veiga AS, Sinthuvanich C, Gaspar D, Franquelim HG, Castanho MA, Schneider JP. Biomaterials. 2012 Sep 17. pii: S0142-9612(12)00949-0. doi: 10.1016/j.biomaterials.2012.08.046. PMID: 22995710
"Anticancer Peptide SVS-1: Efficacy Precedes Membrane Neutralization." Gaspar D, Veiga AS, Sinthuvanich C, Schneider JP, Castanho MA. Biochemistry. 2012 Aug 14;51(32):6263-5. Epub 2012 Aug 3. PMID: 22839778
"Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins." Carvalho FA, Carneiro FA, Martins IC, Assunção-Miranda I, Faustino AF, Pereira RM, Bozza PT, Castanho MA, Mohana-Borges R, Da Poian AT, Santos NC. J Virol. 2012 Feb;86(4):2096-108. Epub 2011 Nov 30. PMID: 22130547
"The Mechanism of Action of Antimicrobial Peptides: Lipid Vesicles vs. Bacteria." Melo MN, Castanho MA. Front Immunol. 2012;3:236. Epub 2012 Aug 2. PMID: 22876247
"Chemical conjugation of the neuropeptide kyotorphin and ibuprofen enhances brain targeting and analgesia." Ribeiro MM, Pinto AR, Domingues MM, Serrano I, Heras M, Bardaji ER, Tavares I, Castanho MA. Mol Pharm. 2011 Oct 3;8(5):1929-40. Epub 2011 Aug 24. PMID: 21830793
"Decoding the Membrane Activity of the Cyclotide Kalata B1. The Importance of Phosphatidiletanolamine Phospholipids and Lipid Organization on Hemolytic and anti-HIV Activities." Henriques, ST, Huang, YH, Rosengren, KJ, Franquelim, HG, Carvalho, FA, Johnson, A, Sonza, S, Tachedjian, G, Castanho, MARB, Daly, NL, Craik, DJ. Journal of Biological Chemistry. 286:27, 24231-24241, DOI: 10.1074/jbc.M111.253393 (JUL 8 2011)
"Anti-HIV-1 antibodies 2F5 and 4E10 interact differently with lipids to bind their epitopes" Franquelim, HG, Chiantia, S, Veiga, AS, Santos, NC, Schwille, P, Castanho, MARB. AIDS, 25:4, 419-428. DOI: 10.1097/QAD.0b013e328342ff11 (FEB 20 2011)
"Escherichia coli Cell Surface Perturbation and Disruption Induced by Antimicrobial peptides BP100 and pepR". Carla S Alves, Manuel N Melo, Henri G Franquelim, Rafael Ferre, Marta Planas, Lidia Feliu, Eduard Bardají, Wioleta Kowalczyk, David Andreu, Nuno C Santos, Miguel X Fernandes, Miguel A R B Castanho. J Biol Chem (2010) vol. 285 (36) pp. 27536-44
"Antimicrobial peptides: linking partition, activity and high membrane.bound concentrations"; M. N. Melo, R. ferre and M. Castanho (2009) Nature Rev. Microbiology
"How to address CPP and AMP translocation? Methods to detect and quantify peptide internalization in vitro and in vivo"; S. T. Henriques, M. N. Melo, M. A. R. B. Castanho (2007) Molec. Membrane Biol 24, 173-184.
"Cell-penetrating peptides and antimicrobial peptides: how different are they?";S. T. Henriques, M. N. Melo, M A. R. B. Castanho (2006) Biochem. J. 399, 1–7
"Quantifying molecular partition into model systems of biomembranes: an emphasis on optical spectroscopic methods"
N. C. Santos, M. Prieto, M. A. R. B. Castanho (2003) Biochim. Biophys. Acta, 1612, 123-135.

2012

"Arginine-rich self-assembling peptides as potent antibacterial gels." Veiga AS, Sinthuvanich C, Gaspar D, Franquelim HG, Castanho MA, Schneider JP. Biomaterials. 2012 Sep 17. pii: S0142-9612(12)00949-0. doi: 10.1016/j.biomaterials.2012.08.046. PMID: 22995710 PubMed
"Extracellular Alpha-Synuclein Oligomers Modulate Synaptic Transmission and Impair LTP Via NMDA-Receptor Activation."Diógenes MJ, Dias RB, Rombo DM, Vicente Miranda H, Maiolino F, Guerreiro P, Näsström T, Franquelim HG, Oliveira LM, Castanho MA, Lannfelt L, Bergström J, Ingelsson M, Quintas A, Sebastião AM, Lopes LV, Outeiro TF. J Neurosci. 2012 Aug 22;32(34):11750-11762. PMID: 22915117 PubMed
"Phosphatidylethanolamine-binding is a conserved feature of cyclotide-membrane interactions." Henriques ST, Huang YH, Castanho MA, Bagatolli LA, Sonza S, Tachedjian G, Daly NL, Craik DJ. J Biol Chem. 2012 Aug 17. [Epub ahead of print] PMID: 22854971 PubMed
"Anticancer Peptide SVS-1: Efficacy Precedes Membrane Neutralization." Gaspar D, Veiga AS, Sinthuvanich C, Schneider JP, Castanho MA. Biochemistry. 2012 Aug 14;51(32):6263-5. Epub 2012 Aug 3. PMID: 22839778 PubMed
"The disordered N-terminal region of dengue virus capsid protein contains a lipid-droplet-binding motif." Martins IC, Gomes-Neto F, Faustino AF, Carvalho FA, Carneiro FA, Bozza PT, Mohana-Borges R, Castanho MA, Almeida FC, Santos NC, Da Poian AT. Biochem J. 2012 Jun 15;444(3):405-15. PMID: 22428600 PubMed
"Antimicrobial properties of analgesic kyotorphin peptides unraveled through atomic force microscopy." Ribeiro MM, Franquelim HG, Torcato IM, Ramu VG, Heras M, Bardaji ER, Castanho MA. Biochem Biophys Res Commun. 2012 Apr 13;420(3):676-9. Epub 2012 Mar 17. PMID: 22450328 PubMed
"Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins." Carvalho FA, Carneiro FA, Martins IC, Assunção-Miranda I, Faustino AF, Pereira RM, Bozza PT, Castanho MA, Mohana-Borges R, Da Poian AT, Santos NC. J Virol. 2012 Feb;86(4):2096-108. Epub 2011 Nov 30. PMID: 22130547 PubMed
"The Mechanism of Action of Antimicrobial Peptides: Lipid Vesicles vs. Bacteria." Melo MN, Castanho MA. Front Immunol. 2012;3:236. Epub 2012 Aug 2. PMID: 22876247
PubMed

2011

"Chemical conjugation of the neuropeptide kyotorphin and ibuprofen enhances brain targeting and analgesia." Ribeiro MM, Pinto AR, Domingues MM, Serrano I, Heras M, Bardaji ER, Tavares I, Castanho MA. Mol Pharm. 2011 Oct 3;8(5):1929-40. Epub 2011 Aug 24. PMID: 21830793 PubMed
"Decoding the Membrane Activity of the Cyclotide Kalata B1. The Importance of Phosphatidiletanolamine Phospholipids and Lipid Organization on Hemolytic and anti-HIV Activities." Henriques, ST, Huang, YH, Rosengren, KJ, Franquelim, HG, Carvalho, FA, Johnson, A, Sonza, S, Tachedjian, G, Castanho, MARB, Daly, NL, Craik, DJ. Journal of Biological Chemistry. 286:27, 24231-24241, DOI: 10.1074/jbc.M111.253393 (JUL 8 2011) PubMed
"Inhibition of nociceptive responses after systemic administration of amidated kyotorphin" Ribeiro, MMB, Pinto, A, Pinto, M, Heras, M, Martins, I, Correia, A, Bardaji, E, Tavares, I, Castanho, M. British Journal of Pharmacology, 163:5, 964-973 DOI: 10.1111/j 1476-5381.2011.01290.x (JUL 2011) Wiley
"Using Zeta-potential Measurements to Quantify Peptide Partition to Lipid Membranes"Freire, JM, Domingues, MM, Matos, J, Melo, MN, Veiga, AS, Santos, NC, Castanho, MARB, Eur Biophys J 40:4, SI:481-487, DOI: 10.1007/s00249-010-0661-4 (APR 2011) PubMed
"Anti-HIV-1 antibodies 2F5 and 4E10 interact differently with lipids to bind their epitopes" Franquelim, HG, Chiantia, S, Veiga, AS, Santos, NC, Schwille, P, Castanho, MARB. AIDS, 25:4, 419-428. DOI: 10.1097/QAD.0b013e328342ff11 (FEB 20 2011)
PubMed

2010

"The role of blood cell membrane lipids on the mode of action of HIV-1 fusion inhibitor sifuvirtide." Matos PM, Freitas T, Castanho MA, Santos NC. Biochem Biophys Res Commun. 2010 Dec 17;403(3-4):270-4. Epub 2010 Nov 12. PubMed
"In vitro blood-brain barrier models--latest advances and therapeutic applications in a chronological perspective." Ribeiro MM, Castanho MA, Serrano I. Mini Rev Med Chem. 2010 Mar;10(3):262-70. PubMed
"HIV-1 fusion inhibitor peptides enfuvirtide and T-1249 interact with erythrocyte and lymphocyte membranes." Matos PM, Castanho MA, Santos NC. PLoS One. 2010 Mar 23;5(3):e9830. PubMed
"Unravelling the molecular basis of the selectivity of the HIV-1 fusion inhibitor sifuvirtide towards phosphatidylcholine-rich rigid membranes." Franquelim HG, Veiga AS, Weissmüller G, Santos NC, Castanho MA. Biochim Biophys Acta. 2010 Jun;1798(6):1234-43. Epub 2010 Feb 11. PubMed
"Fast Membrane Assiciation is Crucial factor in the peptide pep-1 Translocation mechanism: a kinetic study by surface plasmon resonance" Henriques ST, Castanho MA, Pattenden LK, Aquilar MI. Biopolymers. 2010; 94 (3): 314-22
PubMed
"Is PrP (106-126) fragment involved in the membrane activity of the Prion protein?". Henriques ST, Castanho MA. Curr Protein Pept Sci. 2010 Aug; 11(5) 326-33
PubMed
"Drug-lipid interaction evaluation: why a 19th century solution?". Ribeiro MMB, Melo MN, Serrano ID, Santos NC and Castanho MARB (2010) Trends in Pharmacological Sciences (2010) vol. 31 pp. 449-454
PubMed
"Quantitative assessment of peptide-lipid interactions. Ubiquitous fluorescence methodologies." Pedro M Matos, Henri G Franquelim, Miguel A R B Castanho, Nuno C Santos. Biochim Biophys Acta (2010) vol. 1798 (11) pp. 1999-2012
PubMed
"Escherichia coli Cell Surface Perturbation and Disruption Induced by Antimicrobial peptides BP100 and pepR". Carla S Alves, Manuel N Melo, Henri G Franquelim, Rafael Ferre, Marta Planas, Lidia Feliu, Eduard Bardají, Wioleta Kowalczyk, David Andreu, Nuno C Santos, Miguel X Fernandes, Miguel A R B Castanho. J Biol Chem (2010) vol. 285 (36) pp. 27536-44
PubMed
"Cyclotides as templates in drug design."Henriques ST and Craik DJ (2010) Drug Discov Today 15, 57-64. PubMed

2009

"rBPI₂₁ Promotes Lipopolysaccharide Aggregation and exerts Its Antimicrobial Effects by (Hemi)fusion of PG.Containing membranes.", M. M. Domingues, M. A. R. B. Castanho and N. C. Santos (2009) PLoS ONE, 4 PubMed
“Interaction of the dengue virus fusion peptide with membranes assessed by NMR: The essential role of the envelope protein TRP101 for membrane fusion.”, Melo MN, Sousa FJ, Carneiro FA, Castanho MA, Valente AP, Almeida FC, Da Poian AT, Mohana-Borges R. (2009) J Mol Biol. PubMed
“Interactions of HIV-1 antibodies 2F5 and 4E10 with a gp41 epitope prebound to host and viral membrane model systems” Ana S. Veiga, Leonard K. Pattenden, Jordan M. Fletcher, Miguel A. R. B. Castanho and Marie Isabel Aguilar (2009) ChemBioChem, 10, 1032–1044 PubMed
"The Toxicity of Prion Protein Fragment PrP (106-126) is Not Mediated by Membrane Permeabilization as Shown by M112W substituition"; S. T. Henriques, L. K. Pattenden, M. I. Aguilar and M. A. R. B. Castanho (2009) Biochemistry, 48, 4198-4208
PubMed
Free PDF
"Antimicrobial peptides: linking partition, activity and high membrane.bound concentrations"; M. N. Melo, R. ferre and M. Castanho (2009) Nature Rev. Microbiology PubMed
"Synergistic effects of the membrane actions of cecropin-melitin antimicrobial hybrid peptide BP100"; R. Ferre, M. N. Melo, A. D. Correia, L. Feliu, E. Bardají, M. Planas and M. castanho (2009) Biophys. J. PubMed
"Fold-Unfold transitions in the selectivity and mechanism of action of the N-terminal fragment of the Bactericidal/Permeability-Increasing protein (rBPI21)"; M. M. Domingues, S. C. D. N. Lopes, N. C. Santos, A. Quintas and M. A. R. B. Castanho (2009), Biophys. J. PubMed

2008 "PrP(106-126) does not interact with membranes under physiological conditions." Henriques ST, Pattenden LK, Aguilar MI, Castanho MA. Biophys J. 2008 Aug;95(4):1877-89. Epub 2008 May 9
PubMed
"Sifuvirtide screens rigid membrane surfaces. establishment of a correlation between efficacy and membrane domain selectivity among HIV fusion inhibitor peptides."Franquelim HG, Loura LM, Santos NC, Castanho MA. J Am Chem Soc. 2008 May 14;130(19):6215-23. Epub 2008 Apr 15
PubMed
PubMed
“What can light scattering spectroscopy do for membrane-active peptide studies?”; M. M. Domingues, P. S. Santiago, M.A.R.B. Castanho, N. C. Santos (2008) J. Pept. Sci., 14, 394-400. PubMed
“Enfuvirtide effects on human erythrocytes and lymphocytes functional properties.”; C. R. Vieira, M. Castanho, C. Saldanha, N. C. Santos (2008) J. Pept. Sci., 14, 448-454 PubMed
"Translocation or membrane disintegration? Implication of peptide-membrane interactions in pep-1 activity." S.T. Henriques, M. Castanho (2008) J. Pept. Sci., 482-487 PubMed
2007“Molecular interaction studies of peptides using steady-state fluorescence intensity. Static (de)quenching revisited.”; M. M. Ribeiro, H. Franquelim, M. Castanho, A. S. Veiga (2007) J. Pept. Sci., 14, 401-406 PubMed
"Hepatitis C virus core protein binding to lipid membranes. The role of domains 1 and 2." Ana J. Pérez-Berná, Ana Salomé Veiga, Miguel A.R.B. Castanho, and José Villalaín (2007) J. Viral Hepatitis, 15, 346-356 PubMed
"The influence of cholesterol on the interaction of HIV gp41 membrane proximal region-derived peptides with lipid bilayers"; A.S. Veiga, M. Castanho (2007) FEBS J, 274,5096-5104 PubMed
"How to address CPP and AMP translocation? Methods to detect and quantify peptide internalization in vitro and in vivo"; S. T. Henriques, M. N. Melo, M. A. R. B. Castanho (2007) Molec. Membrane Biol 24, 173-184. PubMed
Energy-independent translocation of cell-penetrating peptides occurs without formation of pores. A biophysical study with pep-1."; S.T. Henriques, A. Quintas, L. A. Bagatolli, F. Homblé, M.A.R.B. Castanho (2007) Molec. Membrane Biol 24, 282-293. PubMed
"Omiganan interaction with bacterial membranes and cell wall models. Assigning a biological role to saturation."; M. N. Melo, M.A.R.B. Castanho (2007) Biochimica et Biophysica Acta 1768, 1277–1290 PubMed

2006"Cell-penetrating peptides and antimicrobial peptides: how different are they?";S. T. Henriques, M. N. Melo, M A. R. B. Castanho (2006) Biochem. J. 399, 1–7
PubMed
PDF
“Chiral Recognition of D-Kyotorphin by Lipidic Membranes: Relevance Toward Improved Analgesic Efficiency”; S. C. D. N. Lopes, A. Fedorov, M. A. R. B. Castanho (2006) ChemMedChem 1, 723-728.
PubMed
PDF
“The membranes’ role in the HIV-1 neutralizing monoclonal antibody 2F5 mode of action needs re-evaluation”; A. S. Veiga, M. A.R.B. Castanho (2006) Antiviral Res. 71, 69-72.
PubMed
PDF
“Omiganan pentahydrochloride in the front line of clinical applications of antimicrobial peptides”; M. N. Melo, D. Dugourd, M. A. R. B. Castanho (2006) Recent Patent Rev. Anti-infective Drug Discovery 1, 201-207.
PubMed
"Conformational and Orientational Guidance of the Analgesic Dipeptide Kyotorphin Induced by Lipidic Membranes: Putative Correlation toward Receptor Docking”;S. C. D. N. Lopes, C. M. Soares, A. M. Baptista, E. Goormaghtigh, B. J. Costa Cabral, M. A. R. B. Castanho (2006) J. Phys. Chem. B 110, 3385-3394.
PubMed
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"Why are HIV-1 fusion inhibitors not effective against SARS-CoV? Biophysical evaluation of molecular interactions" S. Veiga, Y. Yuan, X. Li, N. Santos, G. Liu, M. Castanho (2006) BBA - Gen Subjects, 1760, 55 - 61 PDF

2005"Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptides.A fluorescence study using Pep-1."; S. T. Henriques, J. Costa; M. A. R. B. Castanho (2005) FEBS Lett.,4498-4502. PDF available
"Overview of Common Spectroscopic Methods to Determine the Orientation/Alignment of Membrane Probes and Drugs in Lipidic Bilayers"; S. Lopes, M. A. R. B. Castanho (2005) Curr. Org.Chemistry, 9; 889-898. PDF available
"Translocation of b-Galactosidase Mediated by the Cell-Penetrating Peptide Pep-1 into Lipid Vesicles and Human HeLa Cells Is Driven by Membrane Electrostatic Potential"; S. T. Henriques, J. Costa; M. A. R. B. Castanho (2005) Biochemistry, 10189-10198. PDF available
"Environmental factors that enhance the action of the cell-penetrating peptide pep-1. A spectroscopic study using lipidic vesicles"; S. T. Henriques, M. A. R. B. Castanho (2005) Biochim. Biophys. Acta - Biomembranes, 1669, 75- 86 PDF available
"Lipidic membranes are potential "catalysts" in the ligand activity of the multifunctional pentapeptide neo-kyotorphin"; S. Lopes, A. Fedorov, M. A. R. B. Castanho (2005) ChemBioChem, 6, 697 - 702. PDF available

2004"Cholesterol modulates maculosins orientation in model systems of biological membranes. Relevance towards putative molecular recognition"; S. Lopes, A. fedorov, M. A. R. B. Castanho (2004) Steroids, 69, 825-830 PDF available
"HIV fusion inhibitor peptide T1249 is able to insert or adsorb to lipidic bilayers. Putative correlation with improved efficiency"
S. Veiga, N. Santos, L. Loura, A. Fedorov, M. Castanho (2004) J. Am. Chem. Soc., 126, 14758-14763.
PDF available
"Consequences of nonlytic membrane perturbation to the translocation of the cell penetrating peptide Pep-1 in lipidic vesicles"
S. T Henriques, M. Castanho (2004) Biochemistry, 43, 9716-9724.
PDF Available
"Filipin orientation revealed by linear dichroism. Implication for a model os action"
S. Lopes, E. Goormaghtigh, B. J. Costa Cabral, M. Castanho (2004) J. Am. Chem. Soc., 126, 5396-5402.
PDF available
"Putative role of membranes in the HIV fusion inhibitor enfuvirtide mode of action at the molecular level"
S. Veiga, S. Henriques, N. Santos, M. Castanho (2004) Biochem. J., 377, 107-110.
PDF Available

2001-2003"Quantifying molecular partition into model systems of biomembranes: an emphasis on optical spectroscopic methods"
N. C. Santos, M. Prieto, M. A. R. B. Castanho (2003) Biochim. Biophys. Acta, 1612, 123-135.
PDF Available
"Using UV-Vis. linear dichroism to study the orientation of molecular probes and biomolecules in lipidic membranes"
M. A. R. B. Castanho, M. X. Fernandes, S. Lopes (2003) Spectroscopy, 17, 377-398.
PDF Available
"Joint Determination by Brownian Dynamics and Fluorescence Quenching of Membrane Biomolecules in-Depth Location Profile."
M. X. Fernandes, J. García de la Torre, M. A. R. B. Castanho (2002) Anal. Biochem., 307, 1-12.
PDF Available
"Revealing the orientation of nystatin and amphotericin B in lipidic multilayers by UV-Vis. linear dichroism."
S. Lopes, M. A. R. B. Castanho (2002) J. Phys. Chem. B, 106, 7278-7282.
PDF Available
"A photophysical study of the polyene antibiotic filipin self-aggregation and filipin-ergosterol interaction."
L. M. S. Loura, M. A. R. B. Castanho, A. Fedorov, M. Prieto (2001) Biochim. Biophys. Acta, 1510, 125-135.
PDF Available

1992-1998
"Interaction of the major epitope region of HIV protein gp41 with membrane model systems. A fluorescence spectroscopy study."
N. C. Santos, M. J. E. Prieto, M. A. R. B. Castanho (1998) Biochemistry, 37, 8674-8682.
PDF Available
"Filipin induced lesions in planar phospholipid bilayers imaged by atomic force microscopy"
N. C. Santos, E. Ter-Ovanesyan, J. A. Zasadzinski, M. J. E. Prieto, M. A. R. B. Castanho (1998) Biophys. J., 75, 1858-1868.
PDF Available
"Fluorescence quenching data interpretation in biological systems"
M. A. R. B. Castanho, M. J. E. Prieto (1998) Biochim. Biophys. Acta, 1373, 1-16.
PDF Available
"Separating the turbidity spectra of vesicles from the absorption spectra of membrane probes and other chromophores"
M. A. R. B. Castanho, N. C. Santos, L. M. S. Loura (1997) Eur. Biophys. J., 26, 253-259.
PDF available
"Structural characterization of organized systems of polysaccharides and phospholipids by light scattering spectroscopy and electron microscopy"
N. C. Santos, A. Sousa, D. Betbeder, M. J. E. Prieto, M. A. R. B. Castanho (1997) Carbohydr. Res., 300, 31-40.
PDF available
"Filipin Fluorescence Quenching by Spin-Labeled Probes: Studies in Aqueous Solution and in a Membrane Model System"
M. A. R. B. Castanho, M. Prieto (1995) Biophys. J., 69, 155-168.
PDF available
"Rod-like cholesterol micelles in aqueous solution studied using polarized and depolarized dynamic light scattering"
M. A. R. B. Castanho, W. Brown, M. Prieto (1992) Biophys. J. , 63, 1455-1461.
PDF available

Av. Professor Egas Moniz
Edifício Egas Moniz,
1649-028 Lisboa
Portugal

Tel: (+351)217985136
Fax: (+351)217999477

Editor

Book Chapters