2012

M.M. Domingues, N.C. Santos, M.A.R.B. Castanho (2012) Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies. Curr. Protein Pept. Sci., in press.

M.M. Ribeiro, M.M. Domingues, J.M Freire, N.C. Santos, M.A.R.B. Castanho (2012) Translocating the blood-brain barrier using electrostatics. Front. Cell Neurosci., 6, 44.

S. Padilla-Parra, P.M. Matos, N. Kondo, M. Marin, N.C. Santos, G.B. Melikyan (2012) Quantitative imaging of endosome acidification and single retrovirus fusion with distinct pools of early endosomes. Proc. Natl. Acad. Sci. USA, 109, 17627-32.

N.C. Santos (2012) Portugal´s Age of Enlightenment. Science, 335, 401-402.

M. Rodrigues, A. Santos, B.G. de la Torre, G. Radis-Baptista, D. Andreu, N.C. Santos (2012) Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes. Biochim. Biophys. Acta., 1818, 2707-2717.

S. Goncalves, A. Teixeira, J. Abade, L.N. de Medeiros, E. Kurtenbach, N.C. Santos (2012) Evaluation of the membrane lipid selectivity of the pea defensin Psd1. Biochim. Biophys. Acta., 1818, 1420-1426.

A.P. Martins, P.A. Lopes, M.S. Madeira, S.V. Martins, N.C. Santos, T.F. Moura, J.A. Prates,G. Soveral (2012) Differences in lipid deposition and adipose membrane biophysical properties from lean and obese pigs under dietary protein restriction. Biochem. Biophys. Res. Commun., 423, 170-175.

I.C. Martins, F. Gomes-Neto, A.F. Faustino, F.A. Carvalho, F.A. Carneiro, P.T. Bozza, R. Mohana-Borges, M.A. Castanho, F.C. Almeida, N.C. Santos, A.T. Da Poian (2012) The disordered N-terminal region of dengue virus capsid protein contains a lipid-droplet-binding motif. Biochem J 444, 405-415.

F.A. Carvalho, N.C. Santos (2012) Atomic force microscopy-based force spectroscopy-biological and biomedical applications. IUBMB Life, 64, 465-472.

F.A. Carvalho, F.A. Carneiro, IC Martins, I. Assunção-Miranda, A.F. Faustino, R.M. Pereira, P.T. Bozza, M.A. Castanho, R. Mohana-Borges, A.T. Da Poian, N.C. Santos (2012) Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins, J. Virol., 86, 2096-108.

P. Eaton, V. Zuzarte-Luis, M.M. Mota, N.C. Santos, M. Prudêncio (2012) Infection by Plasmodium changes shape and stiffness of hepatic cells, Nanomedicine (NBM), 8, 17-19.

2011

A.P. Martins, P.A. Lopes, A.S.H. Costa, S.V. Martins, N.C. Santos, J.A.M. Prates, T.F. Moura, G. Soveral (2011) Differential mesenteric fat deposition in bovines fed on silage or concentrate is independent of membrane permeability to glycerol, Animal, 5, 1949-1956.

M. Rodrigues, B.G. de la Torre, G. Rádis-Baptista, N.C. Santos, D. Andreu (2011) Efficient cellular delivery of β-galactosidase mediated by NrTPs, a new family of cell-penetrating peptides, Bioconjugate Chem., 22, 2339-2344.

H.G. Franquelim, F.F. De-Sousa, A.S. Veiga, N.C. Santos, M.A.R.B. Castanho (2011) Cationic liposomes are possible drug-delivery systems for HIV fusion inhibitor Sifuvirtide, Soft Matter, 7, 11089-11092.

H.G. Franquelim, S. Chiantia, A.S. Veiga, N.C. Santos, P. Schwille, M.A.R.B. Castanho (2011) Anti-HIV-1 antibodies 2F5 and 4E10 interact differently with lipids to bind their epitopes, AIDS, 25, 419-428.

J.M. Freire, M.M. Domingues, J. Matos, M.N. Melo, A.S. Veiga, N.C. Santos, M.A.R.B. Castanho (2011) Using zeta-potential measurements to quantify peptide partition to lipid membranes, Eur. Biophys. J., 40, 481-487.

F.A. Carvalho, S. de Oliveira, T. Freitas, S. Gonçalves, N.C. Santos (2011) Variations on fibrinogen-erythrocyte interactions during cell aging, PLoS ONE, 6, e18167.

A. Ascenso, R. Guedes, R. Bernardino, H. Diogo, F.A. Carvalho, N.C. Santos, A.M. Silva, H.C. Marques (2011) Complexation and full characterization of the tretinoin and dimethyl-β-cyclodextrin complex, AAPS PharmSciTech, 12, 553-563.

M. Castanho, N.C. Santos (2011) Peptide drug discovery and development: Translational research in academia and industry, Wiley-VCH, Weinheim.

N.C. Santos (2010) In and out – biochemical and biophysical mechanisms of viral fusion and assembly [editorial]. TheScientificWorldJournal, 10, 419-421.

A. Santos, A. Duarte, A. Fedorov, J. Martinho and I. Moura (2010) Rubredoxin mutant A51C unfolding dynamics: a Förster Resonance Energy Transfer study. Biophys. Chem., 148, 131-137.

P.S. Santiago, F.A.O. Carvalho, M.M. Domingues, J.W.P. Carvalho, N.C. Santos, M. Tabak (2010) Isoelectric point determination for Glossoscolex paulistus extracellular hemoglobin: Oligomeric stability in acidic pH and relevance to protein-surfactant interactions, Langmuir, 26, 9794-9801.

A.P. Martins, P.A. Lopes, S.V. Martins, A. Madeira, N.C. Santos, J.A.M. Prates, T.F. Moura, G. Soveral (2010) Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats, Biochem. Biophys. Res. Commun., 398, 199–204.

I. Kuperstein, K. Broersen, I. Benilova, J. Rozenski, W. Jonckheere, M. Debulpaep, A. Vandersteen, I. Segers-nolten, K. Van Der Werf, V. Subramaniam, D. Braeken, G. Calewaert, C. Bartic, R. D’Hooge, I.C. Martins, F. Rousseau, J. Schymkowitz and B. De Strooper (2010) Neurotoxicity of Alzheimer disease Aβ-peptides is induced by small changes in the Aβ42 to Aβ40 ratio. EMBO J., 29, 3408-3420.

F.A. Carvalho, S. Connell, G. Miltenberger-Miltenyi, S.V. Pereira, A. Tavares, R.A.S. Ariëns, N.C. Santos (2010) Atomic force microscopy-based molecular recognition of a fibrinogen receptor on human erythrocytes, ACS Nano, 4, 4609-4620.

P.M. Matos, H.G. Franquelim, M.A.R.B. Castanho, N.C. Santos (2010) Quantitative assessment of peptide–lipid interactions. Ubiquitous fluorescence methodologies., Biochim. Biophys. Acta, 1798, 1999-2012.

S. Maurer-Stroh, M. Debulpaep, M.L. De La Paz, I.C. Martins, J. Reumers, A. Copland, L. Serpell, L. Serrano, J. Schymkowitz, K. Morris and F. Rousseau (2010) Exploring the sequence determinants of amyloid structure using position-specific scoring matrices. Nature Methods, 7, 237-2342.

C.S. Alves, M.N. Melo, H.G. Franquelim, R. Ferre, M. Planas, L. Feliu, E. Bardají, W. Kowalczyk, D. Andreu, N.C. Santos, M.X. Fernandes, M.A.R.B. Castanho (2010) Escherichia coli cell surface perturbation and disruption induced by antimicrobial peptides, BP100 and pepR., J. Biol. Chem., 285, 27536-27544.

M.M. Ribeiro, M.N. Melo, I. Serrano, N.C. Santos, M.A.R.B. Castanho (2010) Drug-lipid interaction evaluation: Why a 19th century solution?, Trends Pharmacol. Sci., 31, 449-454.

P.S. Santiago, J.W.P. Carvalho, M.M. Domingues, N.C. Santos, M. Tabak (2010) Thermal stability of extracellular hemoglobin of Glossoscolex paulistus: Determination of activation parameters by optical spectroscopic and differential scanning calorimetric studies, Biophys. Chem., 152, 128-138.

P.M. Matos, T. Freitas, M.A.R.B. Castanho, N.C. Santos (2010) Sifuvirtide interaction with blood cell membranes denotes the conjugation of different lipid preferences, Biochem. Biophys. Res. Commun., 403, 270-274.

P.M. Matos, M.A.R.B. Castanho, N.C. Santos (2010) HIV-1 Fusion Inhibitor Peptides Enfuvirtide and T-1249 Interact with Erythrocyte and Lymphocyte Membranes, PLoS One, 5, e9830.

N.C. Santos, J.M. Silva, T. Freitas, M. Doroana, N. Duarte, L. Tavares, F. Antunes, C. Saldanha (2010) Blood cell membrane fluidity and intracellular Ca2+ changes in antiretroviral-naïve and -treated HIV-1-infected patients, ScientificWorldJournal, 10, 350-355.

H.G. Franquelim, A.S. Veiga, G. Weissmüller, N.C. Santos, M.A. Castanho (2010) Unravelling the molecular basis of the selectivity of the HIV-1 fusion inhibitor sifuvirtide towards phosphatidylcholine-rich rigid membranes, Biochim Biophys Acta, 1798, 1234-1243.

M.M. Domingues, M.A.R.B. Castanho, N.C. Santos (2009) rBPI(21) promotes lipopolysaccharide aggregation and exerts its antimicrobial effects by (hemi)fusion of PG-containing membranes, PLoS One, 4, e8385.

M.M. Domingues, S.C. Lopes, N.C. Santos, A. Quintas, M.A. Castanho (2009) Fold-unfold transitions in the selectivity and mechanism of action of the N-terminal fragment of the bactericidal/permeability-increasing protein (rBPI(21)), Biophys. J., 96, 987-996.

2008

H.G. Franquelim, L.M.S. Loura, N.C. Santos, M.A.R.B. Castanho (2008) Sifuvirtide screens rigid membrane surfaces. Establishment of a correlation between efficacy and membrane domain selectivity among HIV fusion inhibitor peptides, J. Am. Chem. Soc., 130, 6215.

P.S. Santiago, F. Moura, L.M. Moreira, M.M. Domingues, N.C. Santos, M. Tabak (2008) Dynamic light scattering and optical absorption spectroscopy study of pH and temperature stabilities of the extracellular hemoglobin of Glossoscolex paulistus, Biophys. J., 94, 2228-2240.

P.M. Matos, S. Gonçalves, N.C. Santos (2008) Interaction of peptides with biomembranes assessed by potential sensitive fluorescent probes, J. Pept. Sci., 14, 407-415.

C.R. Vieira, M. Castanho, C. Saldanha, N.C. Santos (2008) Enfuvirtide effects on human erythrocytes and lymphocytes functional properties, J. Pept. Sci., 14, 448-454.

M.M. Domingues, P.S. Santiago, M.A.R.B. Castanho, N.C. Santos (2008) What can light scattering spectroscopy do for membrane-active peptide studies?, J. Pept. Sci., 14, 394-400.

S. Gonçalves, N.C. Santos, J. Martins-Silva, C. Saldanha (2007) Fluorescence spectroscopy evaluation of fibrinogen--estradiol binding, J. Photochem. Photobiol. B-Biol., 86, 170-176.

S. Gonçalves, A. Vasco Maria, A.S. Silva-Herdade, J. Martins e Silva, C. Saldanha (2007) Milk enriched with phytosterols reduces plasma cholesterol levels in healthy and hypercholesterolemic subjects, Nutr. Res., 27, 200-205.

C. Saldanha, A.S. Silva, S. Gonçalves, J. Martins e Silva (2007) Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation, Clin. Hemorheol. Microcirc., 36, 183-194.

J. Martins-Silva, N.C. Santos, M. Doroana, N. Duarte, L. Tavares, F. Antunes, C. Saldanha (2006) Changes in blood cells membrane properties in HIV-1 infected patients, AIDS Res. Hum. Retrovir., 22, 849-853.

S. Gonçalves, N.C. Santos, J. Martins-Silva, C. Saldanha (2006) Fibrinogen--estradiol binding studies by fluorescence spectroscopy. Denaturation and pH effects, J. Fluoresc., 16, 207-213.

A.S. Veiga, N.C. Santos, M.A.R.B. Castanho (2006) An insight on the leading HIV entry inhibitors, Recent Patents Anti-Infective Drug Discov., 1, 67-73.

F.A. Carvalho, A.V. Maria, J.M. Braz-Nogueira, J. Guerra, J. Martins-Silva, C. Saldanha (2006) The relation between the erythrocyte nitric oxide and hemorheological parameters, Clin. Hemorheol. Microcirc., 35, 341-347.

A.S. Veiga, Y. Yuan, X. Li, N.C. Santos, G. Liu, M.A.R.B. Castanho (2006) Why are HIV-1 fusion inhibitors not effective against SARS-CoV? Biophysical evaluation of molecular interactions, Biochim. Biophys. Acta, 1760, 55-61.

S. Gonçalves, A. Vasco Maria, A.S. Silva, J. Martins Silva, C. Saldanha (2006) Phytosterols in milk as a depressor of plasma cholesterol levels: Experimental evidence with hypercholesterolemic Portuguese subjects. Clin. Hemorheol. Microcirc., 35, 251-255.

N.C. Santos, J. Martins-Silva, C. Saldanha (2005) Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation, Cell Biochem. Biophys., 43, 419-430.

F.A. Carvalho, L.M. Graça, J. Martins-Silva, C. Saldanha (2005) Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase, FEBS J., 272, 5584-5594.

N.C. Santos, J. Martins-Silva, C. Saldanha (2004) PTEN “meets” DMSO [editorial], Leuk. Res., 29, 361-362.

A.S. Veiga, N.C. Santos, L.M.S. Loura, A. Fedorov, M.A.R.B. Castanho (2004) HIV fusion inhibitor peptide T-1249 is able to insert or adsorb to lipidic bilayers. Putative correlation with improved efficiency, J. Am. Chem. Soc., 126, 14758-14763.

C. Saldanha, N.C. Santos, J. Martins-Silva (2004) A colorimetric process to visualize erythrocyte exovesicles aggregates, Biochem. Mol. Biol. Educ., 32, 250-253.

N.C. Santos, M.A.R.B. Castanho (2004) An overview of the biophysical applications of atomic force microscopy, Biophys. Chem., 107, 133-149.

S. Veiga, S. Henriques, N.C. Santos, M. Castanho (2004) Putative role of membranes in the HIV fusion inhibitor enfuvirtide mode of action at the molecular level, Biochem. J., 377, 107-110.

F.A. Carvalho, R. Mesquita, J. Martins-Silva, C. Saldanha (2004) Acetylcholine and choline effects on erythrocytes nitrites and nitrates levels. J. Appl. Toxicol., 24, 419-427.

F.A. Carvalho, J. Martins-Silva, C. Saldanha (2004) Amperometric measurements of nitric oxide in erythrocytes, Biosens. Bioelectron., 20, 505-508.

N.C. Santos, M. Prieto, M. Castanho (2003) Quantifying molecular partition into model systems of biomembranes. An emphasis on optical spectroscopic methods, Biochim. Biophys. Acta, 1612, 123-135.

N.C. Santos, J. Figueira-Coelho, J. Martins-Silva, C. Saldanha (2003) Multidisciplinary utilization of dimethylsulfoxide: Molecular, cellular and pharmacological aspects, Biochem. Pharmacol., 65, 1035-1041.

N.C. Santos, A.C. Silva, M.A.R.B. Castanho, J. Martins-Silva, C. Saldanha (2003) Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy, ChemBioChem, 4, 96-100.

C. Saldanha, N.C. Santos, J. Martins-Silva (2002) Fluorescent probes DPH, TMA-DPH and C17-HC induce erythrocyte exovesiculation, J. Membr. Biol., 190, 75-82.

N.C. Santos, M. Castanho (2002) Fluorescence spectroscopy methodologies on the study of proteins and peptides. On the 150th anniversary of protein fluorescence, Trends Appl. Spectrosc., 4, 113-125.

N.C. Santos, J. Figueira-Coelho, C. Saldanha, J. Martins-Silva (2002) Biochemical, biophysical and haemorheological effects of dimethylsulphoxide on human erythrocytes calcium loading, Cell Calcium, 31, 183-188.

N.C. Santos, M.X.J.J. Fernandes, M.A.R.B. Castanho (1999) Overcoming angular dependency when teaching light scattering using a spectrofluorometer. The molecular weights of latex beads, J. Chem. Educ., 76, 1259.

N.C. Santos, E. Ter-Ovanesyan, J.A. Zasadzinski, M.A.R.B. Castanho (1998) Reconstitution of phospholipid bilayer by an atomic force microscope tip, Biophys. J., 75, 2119-2120.

N.C. Santos, E. Ter-Ovanesyan, J.A. Zasadzinski, M. Prieto, M.A.R.B. Castanho (1998) Filipin-induced lesions in planar phospholipid bilayers imaged by atomic force microscopy, Biophys. J., 75, 1869-1873.

N.C. Santos, M. Prieto, M.A.R.B. Castanho (1998) Interaction of the major epitope region of HIV protein gp41 with membrane model systems. A fluorescence spectroscopy study., Biochemistry, 37, 8674-8682.

M.X.J.J. Fernandes, N.C. Santos, M.A.R.B. Castanho (1998) Continuous particle size distribution analysis with dynamic light scattering. MAXAMPER: A regularization method using the maximum amplitude for the average error and the Lagrange’s multipliers method, J. Biochem. Biophys. Methods, 36, 101-118.

M.A.R.B. Castanho, N.C. Santos, L.M.S. Loura (1997) Separating the turbidity spectra of vesicles from the absorption spectra of membrane probes and other chromophores, Eur. Biophys. J., 26, 253-259.

N.C. Santos, A.M.A. Sousa, D. Betbeder, M. Prieto, M.A.R.B. Castanho (1997) Structural characterization of organized systems of polysaccharides and phospholipids by light scattering spectroscopy and electron microscopy, Carbohydr. Res., 300, 31-40.

N.C. Santos, M.J.E. Prieto, A. Morna-Gomes, D. Betbeder, M.A.R.B. Castanho (1997) Structural characterization (shape and dimensions) and stability of polysaccharide/ lipid nanoparticles, Biopolymers, 41, 511-520.

N.C. Santos, M.A.R.B. Castanho (1996) Teaching light scattering spectroscopy: The dimension and shape of tobacco mosaic virus, Biophys. J., 71, 1641-1650.