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Recent Selected Publications

(last update: June 2015)

 

Vigant F, Santos NC, Lee B (2015) Broad-spectrum antivirals against viral fusion, Nat Rev Microbiol, 13, 426-437, DOI: 10.1038/nrmicro3475 (IF: 23.574).

Faustino AF, Carvalho FA, Martins IC, Castanho MARB, Mohana-Borges R, Almeida FCL, Da Poian AT, Santos NC (2014) Dengue virus capsid protein interacts specifically with very low density lipoproteins, Nanomedicine (NBM), 10, 247-255, DOI: 10.1016/j.nano.2013.06.004 (IF: 6.155).

Hauser CAE, Maurer-Stroh S, Martins IC (2014) Amyloid-based nanosensors and nanodevices. Chem. Soc. Rev., 43, 5326-5345, DOI: 10.1039/C4CS00082J (IF: 33.383).

Padilla-Parra S, Matos PM, Kondo N, Marin M, Santos NC, Melikyan GB (2012) Quantitative imaging of endosome acidification and single retrovirus fusion with distinct pools of early endosomes. Proc. Natl. Acad. Sci. U. S. A. 109, 17627-17632, DOI: 10.1073/pnas.1211714109 (IF: 9.674).

Carvalho FA, Connell S, Miltenberger-Miltenyi G, Pereira SV, Tavares A, Ariëns RAS, Santos NC (2010) Atomic force microscopy-based molecular recognition of a fibrinogen receptor on human erythrocytes. ACS Nano 4, 4609-4620, DOI: 10.1021/nn1009648 (IF: 12.881).

 

Patents

Martins IC, Santos NC, Almeida FCL, Da Poian AT (2012). DENV-derived peptides for the inhibition of the Flavivirus replication. International publication no. WO/2012/159187.
 
Faustino AF, Carvalho FA, Santos NC (2012). Dengue-virus capsid protein-lipoproteins interactions. Instituto Nacional de Propriedade Industrial (INPI), provisional request nr. 20121000044673.

Faustino AF, Martins IC, Almeida FC, Santos NC, Da Poian AT (2014). Understanding dengue virus capsid protein disordered N-terminus and pep14-23-based inhibition. Instituto Nacional de Propriedade Industrial (INPI), provisional request nr. 108040. 

 

Publication List

(last update: June 2015)

 2015

F. Vigant, N.C. Santos, B. Lee (2015). Broad-spectrum antivirals against viral fusion, Nat Rev Microbiol, 13, 426-437 (DOI: 10.1038/nrmicro3475).

J. Freire, N.C. Santos, A.S. Veiga, A.T. Da Poian, M.A.R.B. Castanho (2015). Rethinking the capsid proteins of enveloped viruses: multifunctionality from genome packaging to genome transfection, FEBS J, 282, 2267-2278 (DOI: 10.1111/febs.13274).

A. Hollmann, S. Gonçalves, M.T. Augusto, M.A. Castanho, B. Lee, N.C. Santos (2015). Effects of singlet oxygen generated by a broad-spectrum viral fusion inhibitor on membrane nanoarchitecture, Nanomedicine (NBM), 11, 1163-1167 (DOI: 10.1016/j.nano.2015.02.014).

B.M. Bravo-Ferrada, S. Gonçalves, L. Semorile, N.C. Santos, E.E. Tymczyszyn, A. Hollmann (2015). Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration, J Appl Microbiol, 118, 1409-1417 (DOI: 10.1111/jam.12796).

J.F. Viana, J. Carrijo, C.G. Freitas, A. Paul, J. Alcaraz, C.C. Lacorte, L. Migliolo, C.A. Andrade, R. Falcão, N.C. Santos, S. Gonçalves, A.J. Otero-González, A. Khademhosseini, S.C. Dias, O.L. Franco (2015). Antifungal nanofibers made by controlled release of sea animal derived peptide, Nanoscale, 7, 6238-6246 (DOI: 10.1039/c5nr00767d).

A.F. Faustino, G.M. Guerra, R.G. Huber, A. Hollmann, M.M. Domingues, G.M. Barbosa, F.J. Enguita, P.J. Bond, M.A.R.B. Castanho, A.T. Da Poian, F.C.L. Almeida, N.C. Santos, I.C. Martins (2015). Understanding Dengue Virus Capsid Protein Disordered N‑Terminus and pep14-23-Based Inhibition. ACS Chem Biol, 10, 517-526 (DOI: 10.1021/cb500640t).

M. Rodrigues, D. Andreu, N.C. Santos (2015) Uptake and cellular distribution of nucleolar targeting peptides (NrTPs) in different cell types, Biopolymers (Pept. Sci.), 104, 101-109 (DOI: 10.1002/bip.22610).

 

2014

C.A.E. Hauser, S. Maurer-Stroh, I.C. Martins (2014) Amyloid-based nanosensors and nanodevices. Chem. Soc. Rev., 43, 5326-5345 (DOI: 10.1039/C4CS00082J).

M.T. Augusto, A. Hollmann, M.A.R.B. Castanho, M. Porotto, A. Pessi, N.C. Santos (2014) Improvement of the HIV fusion inhibitor C34 efficacy by membrane anchoring and enhanced exposure. J. Antimicrob. Chemother., 69, 1286-1297 (DOI: 10.1093/jac/dkt529).

M.M. Domingues, P.M. Silva, H.G. Franquelim, F.A. Carvalho, M.A.R.B.Castanho, N.C. Santos (2014) Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria. Nanomedicine (NBM), 10, 543-551 (DOI: 10.1016/j.nano.2013.11.002).

P.M. Silva, S. Gonçalves, N.C. Santos (2014) Defensins: antifungal lessons from eukaryotes, Front. Microbiol., 5, 97 (DOI: 10.3389/fmicb.2014.00097).

A. Hollmann, M.A.R.B. Castanho, B. Lee, N.C. Santos (2014) Singlet oxygen effects on lipid membranes: implication on broad range viral fusion inhibitors mechanism of action. Biochem J., 459, 161-170 (DOI: 10.1042/BJ20131058).

P.M. Matos, D. Andreu, N.C. Santos, R. Gutiérrez-Gallego. (2014) Structural requirements of glycosaminoglycans for their interaction with HIV-1 envelope glycoprotein gp120. Arch. Virol., 159, 555-560 (DOI: 10.1007/s00705-013-1831-3).

J.M. Freire, A.S. Veiga, I. Rego de Figueiredo, B.G. de la Torre, N.C. Santos, D. Andreu, A.T. Da Poian, M.A.R.B. Castanho. (2014) Nucleic acid delivery by cell penetrating peptides derived from dengue virus capsid protein: design and mechanism of action. FEBS J., 281, 191-215 (DOI: 10.1111/febs.12587).

F. Vigant, A. Hollmann, J. Lee, N.C. Santos, M.E. Jung, B. Lee (2014) The rigid amphipathic fusion inhibitor dUY11 acts through photosensitization of viruses. J. Virol., 88, 1849-1853 (DOI: 10.1128/JVI.02907-1).

A.M. Bouchet, N.B. Iannucci, M.B. Pastrian, O. Cascone, N.C. Santos, E.A. Disalvo, A. Hollmann (2014) Biological activity of antibacterial peptides matches synergism between electrostatic and non electrostatic forces. Colloids Surf. B - Biointerfaces, 114, 363-371 (DOI: 10.1016/j.colsurfb.2013.10.025).

A.F. Faustino, F.A. Carvalho, I.C. Martins, M.A.R.B. Castanho, R. Mohana-Borges, F.C.L. Almeida, A.T. Da Poian, N.C. Santos (2014) Dengue virus capsid protein interacts specifically with very low density lipoproteins, Nanomedicine (NBM), 10, 247-255 (DOI: 10.1016/j.nano.2013.06.004).

 

2013

F.A. Carvalho, I.C. Martins, N.C. Santos (2013) Atomic force microscopy and force spectroscopy on the assessment of protein folding and functionality. Arch. Biochem. Biophys., 531, 116-127.

J.M. Freire, A. S. Veiga, B.G. de la Torre, N.C. Santos, D. Andreu, A.T. Da Poian, M.A.R.B. Castanho (2013) Peptides as models for the structure and function of viral capsid proteins: insights on dengue virus capsid. Biopolymers (Pept. Sci.), 100, 325-336.

J. Pimenta, A. Viegas, J. Sardinha, I.C. Martins, E. Cabrita, C. Fontes, J Prates., R. Pereira (2013). NMR Solution Structure and SRP54M predicted interaction of the N-Terminal sequence (1-30) of the ovine Doppel protein. Peptides, 49, 32-40.

M.M. Domingues, M.L. Bianconi, L.R.S. Barbosa, P.S. Santiago, M. Tabak, M.A.R.B. Castanho, R. Itri, N.C. Santos (2013) rBPI21 interacts with negative membranes endothermically promoting the formation of rigid multilamellar structures, Biochim. Biophys. Acta, 1828, 2419-2427.

J.M. Freire, A.S. Veiga, B.G. de la Torre, N.C. Santos, D. Andreu, A.T. Da Poian, M.A.R.B. Castanho (2013) Peptides as models for the structure and function of viral capsid proteins: insights on dengue virus capsid, Biopolymers (Pept. Sci.), 100, 325-336.

A. Ascenso, M. Cruz, C. Euletério, F.A. Carvalho, N.C. Santos, H.C. Marques, S. Simões (2013) Novel tretinoin formulations: a drug-in-cyclodextrin-in-liposome approach, J. Liposome Res., 23, 211-219.

J. Pimenta, A. Viegas, J. Sardinha, A. Santos, I.C. Martins, C. Cantante, F. Dias, R. Soares, E. Cabrita, J. Gonçalves, C. Fontes, J. Prates, R. Pereira (2013). Paving the way through prion-like biogenesis. Ovine Doppel co-expression with SUMO fusion technology, and NMR solution structure of its N-terminal sequence (1-30). Reprod Biol Endocrin, 11:25.

M. Rodrigues, B.G. de la Torre, D. Andreu, N.C. Santos (2013) Kinetic uptake profiles of cell penetrating peptides in lymphocytes and monocytes, Biochim. Biophys. Acta, 1830, 4554-4563.

P.M. Matos, M. Marin, B. Ahn, W. Lam, N.C. Santos, G.B. Melikyan (2013) Anionic lipids are required for Vesicular Stomatitis Virus G protein-mediated single particle fusion with supported lipid bilayers, J. Biol. Chem., 288, 12416-12425.

H.G. Franquelim, D. Gaspar, A.S. Veiga, N.C. Santos, M.A.R.B. Castanho (2013) Decoding distinct membrane interactions of HIV-1 fusion inhibitors using a combined atomic force and fluorescence microscopy approach, Biochim. Biophys. Acta, 1828, 1777-1785.

F. Vigant, J. Lee, A. Hollmann, L.B. Tanner, Z.A. Ataman, T. Yun, G. Shui, H.C. Aguilar, D. Zhang, D. Meriwether, G. Roman-Sosa, L.R. Robinson, T.L. Juelich, H. Buczkowski, S. Chou, M.A.R.B. Castanho, M.C. Wolf, J.K. Smith, A. Banyard, M. Kielian, S. Reddy, M.R. Wenk, M. Selke, N.C. Santos, A.N. Freiberg, M.E. Jung, B. Lee (2013) A mechanistic paradigm for broad-spectrum antivirals that target virus-cell fusion, PLoS Pathog., 9, e1003297.

A. Hollmann, P.M. Matos, M.T. Augusto, M.A.R.B. Castanho, N.C. Santos (2013) Conjugation of cholesterol to HIV-1 fusion inhibitor C34 increases peptide-membrane interactions potentiating its action, PLoS ONE, 8, e60302.

B. Bravo-Ferrada, A. Hollmann, L. Delfederico, D. Valdés La Hens, A. Caballero, L. Semorile. (2013) Patagonian red wines: Selection of Lactobacillus plantarum isolates as potential starter cultures for malolactic fermentation, World J Microbiol Biotechnol, 29, 1537-1549

E.A. Disalvo, A. Hollmann, L. Semorile, F. Martini. (2013) Evaluation of the Defay-Prigogine model for the membrane interphase in relation to biological response in membrane-protein interactions, Biochim. Biophys. Acta, 1828, 1834-1839.

 

2012

I. Gomes, M.J. Feio, N.C. Santos, P. Eaton, A.P. Serro, B. Saramago, E. Pereira, R. Franco (2012) Controlled adsorption of cytochrome c to nanostructured gold surfaces, J. Nanopart. Res., 14, 1321.

A. Hollmann, M. Saviello, L. Delfederico, et al., (2012) Tight controlled expression and secretion of Lactobacillus brevis SlpA in Lactococcus lactis. Biotechnology Letters, 34, 1275–1281.

M.A. Frías, G. Contis, A. Hollmann, E.A. Disalvo (2012) Coordination forces between lipid bilayers produced by ferricyanide and Ca+2. Colloids and Surfaces B: Biointerfaces. 91, 26-33.

M.M. Domingues, N.C. Santos, M.A.R.B. Castanho (2012) Antimicrobial peptide rBPI21: a translational overview from bench to clinical studies, Curr. Protein Pept. Sci., 13, 611‑619.

M.M. Ribeiro, M.M. Domingues, J.M Freire, N.C. Santos, M.A.R.B. Castanho (2012) Translocating the blood-brain barrier using electrostatics. Front. Cell Neurosci., 6, 44.

S. Padilla-Parra, P.M. Matos, N. Kondo, M. Marin, N.C. Santos, G.B. Melikyan (2012) Quantitative imaging of endosome acidification and single retrovirus fusion with distinct pools of early endosomes. Proc. Natl. Acad. Sci. USA, 109, 17627-32.

N.C. Santos (2012) Portugal´s Age of Enlightenment. Science, 335, 401-402.

M. Rodrigues, A. Santos, B.G. de la Torre, G. Radis-Baptista, D. Andreu, N.C. Santos (2012) Molecular characterization of the interaction of crotamine-derived nucleolar targeting peptides with lipid membranes. Biochim. Biophys. Acta, 1818, 2707-2717.

S. Goncalves, A. Teixeira, J. Abade, L.N. de Medeiros, E. Kurtenbach, N.C. Santos (2012) Evaluation of the membrane lipid selectivity of the pea defensin Psd1. Biochim. Biophys. Acta., 1818, 1420-1426.

A.P. Martins, P.A. Lopes, M.S. Madeira, S.V. Martins, N.C. Santos, T.F. Moura, J.A. Prates,G. Soveral (2012) Differences in lipid deposition and adipose membrane biophysical properties from lean and obese pigs under dietary protein restriction. Biochem. Biophys. Res. Commun., 423, 170-175.

I.C. Martins, F. Gomes-Neto, A.F. Faustino, F.A. Carvalho, F.A. Carneiro, P.T. Bozza, R. Mohana-Borges, M.A. Castanho, F.C. Almeida, N.C. Santos, A.T. Da Poian (2012) The disordered N-terminal region of dengue virus capsid protein contains a lipid-droplet-binding motif. Biochem J 444, 405-415.

F.A. Carvalho, N.C. Santos (2012) Atomic force microscopy-based force spectroscopy-biological and biomedical applications. IUBMB Life, 64, 465-472.

F.A. Carvalho, F.A. Carneiro, IC Martins, I. Assunção-Miranda, A.F. Faustino, R.M. Pereira, P.T. Bozza, M.A. Castanho, R. Mohana-Borges, A.T. Da Poian, N.C. Santos (2012) Dengue virus capsid protein binding to hepatic lipid droplets (LD) is potassium ion dependent and is mediated by LD surface proteins, J. Virol., 86, 2096-108.

P. Eaton, V. Zuzarte-Luis, M.M. Mota, N.C. Santos, M. Prudêncio (2012) Infection by Plasmodium changes shape and stiffness of hepatic cells, Nanomedicine (NBM), 8, 17-19.

 

2011

A.P. Martins, P.A. Lopes, A.S.H. Costa, S.V. Martins, N.C. Santos, J.A.M. Prates, T.F. Moura, G. Soveral (2011) Differential mesenteric fat deposition in bovines fed on silage or concentrate is independent of membrane permeability to glycerol, Animal, 5, 1949-1956.

M. Rodrigues, B.G. de la Torre, G. Rádis-Baptista, N.C. Santos, D. Andreu (2011) Efficient cellular delivery of β-galactosidase mediated by NrTPs, a new family of cell-penetrating peptides, Bioconjugate Chem., 22, 2339-2344.

H.G. Franquelim, F.F. De-Sousa, A.S. Veiga, N.C. Santos, M.A.R.B. Castanho (2011) Cationic liposomes are possible drug-delivery systems for HIV fusion inhibitor Sifuvirtide, Soft Matter, 7, 11089-11092.

H.G. Franquelim, S. Chiantia, A.S. Veiga, N.C. Santos, P. Schwille, M.A.R.B. Castanho (2011) Anti-HIV-1 antibodies 2F5 and 4E10 interact differently with lipids to bind their epitopes, AIDS, 25, 419-428.

J.M. Freire, M.M. Domingues, J. Matos, M.N. Melo, A.S. Veiga, N.C. Santos, M.A.R.B. Castanho (2011) Using zeta-potential measurements to quantify peptide partition to lipid membranes, Eur. Biophys. J., 40, 481-487.

F.A. Carvalho, S. de Oliveira, T. Freitas, S. Gonçalves, N.C. Santos (2011) Variations on fibrinogen-erythrocyte interactions during cell aging, PLoS ONE, 6, e18167.

A. Ascenso, R. Guedes, R. Bernardino, H. Diogo, F.A. Carvalho, N.C. Santos, A.M. Silva, H.C. Marques (2011) Complexation and full characterization of the tretinoin and dimethyl-β-cyclodextrin complex, AAPS PharmSciTech, 12, 553-563.

M. Castanho, N.C. Santos (2011) Peptide drug discovery and development: Translational research in academia and industry, Wiley-VCH, Weinheim.

 

2010

N.C. Santos (2010) In and out – biochemical and biophysical mechanisms of viral fusion and assembly [editorial]. TheScientificWorldJournal, 10, 419-421.

A. Santos, A. Duarte, A. Fedorov, J. Martinho and I. Moura (2010) Rubredoxin mutant A51C unfolding dynamics: a Förster Resonance Energy Transfer study. Biophys. Chem., 148, 131-137.

P.S. Santiago, F.A.O. Carvalho, M.M. Domingues, J.W.P. Carvalho, N.C. Santos, M. Tabak (2010) Isoelectric point determination for Glossoscolex paulistus extracellular hemoglobin: Oligomeric stability in acidic pH and relevance to protein-surfactant interactions, Langmuir, 26, 9794-9801.

A.P. Martins, P.A. Lopes, S.V. Martins, A. Madeira, N.C. Santos, J.A.M. Prates, T.F. Moura, G. Soveral (2010) Conjugated linoleic acid reduces permeability and fluidity of adipose plasma membranes from obese Zucker rats, Biochem. Biophys. Res. Commun., 398, 199–204.

I. Kuperstein, K. Broersen, I. Benilova, J. Rozenski, W. Jonckheere, M. Debulpaep, A. Vandersteen, I. Segers-nolten, K. Van Der Werf, V. Subramaniam, D. Braeken, G. Calewaert, C. Bartic, R. D’Hooge, I.C. Martins, F. Rousseau, J. Schymkowitz and B. De Strooper (2010) Neurotoxicity of Alzheimer disease Aβ-peptides is induced by small changes in the Aβ42 to Aβ40 ratio. EMBO J., 29, 3408-3420.

F.A. Carvalho, S. Connell, G. Miltenberger-Miltenyi, S.V. Pereira, A. Tavares, R.A.S. Ariëns, N.C. Santos (2010) Atomic force microscopy-based molecular recognition of a fibrinogen receptor on human erythrocytes, ACS Nano, 4, 4609-4620.

P.M. Matos, H.G. Franquelim, M.A.R.B. Castanho, N.C. Santos (2010) Quantitative assessment of peptide–lipid interactions. Ubiquitous fluorescence methodologies., Biochim. Biophys. Acta, 1798, 1999-2012.

S. Maurer-Stroh, M. Debulpaep, M.L. De La Paz, I.C. Martins, J. Reumers, A. Copland, L. Serpell, L. Serrano, J. Schymkowitz, K. Morris and F. Rousseau (2010) Exploring the sequence determinants of amyloid structure using position-specific scoring matrices. Nature Methods, 7, 237-2342.

C.S. Alves, M.N. Melo, H.G. Franquelim, R. Ferre, M. Planas, L. Feliu, E. Bardají, W. Kowalczyk, D. Andreu, N.C. Santos, M.X. Fernandes, M.A.R.B. Castanho (2010) Escherichia coli cell surface perturbation and disruption induced by antimicrobial peptides, BP100 and pepR., J. Biol. Chem., 285, 27536-27544.

M.M. Ribeiro, M.N. Melo, I. Serrano, N.C. Santos, M.A.R.B. Castanho (2010) Drug-lipid interaction evaluation: Why a 19th century solution?, Trends Pharmacol. Sci., 31, 449-454.

P.S. Santiago, J.W.P. Carvalho, M.M. Domingues, N.C. Santos, M. Tabak (2010) Thermal stability of extracellular hemoglobin of Glossoscolex paulistus: Determination of activation parameters by optical spectroscopic and differential scanning calorimetric studies, Biophys. Chem., 152, 128-138.

P.M. Matos, T. Freitas, M.A.R.B. Castanho, N.C. Santos (2010) Sifuvirtide interaction with blood cell membranes denotes the conjugation of different lipid preferences, Biochem. Biophys. Res. Commun., 403, 270-274.

P.M. Matos, M.A.R.B. Castanho, N.C. Santos (2010) HIV-1 Fusion Inhibitor Peptides Enfuvirtide and T-1249 Interact with Erythrocyte and Lymphocyte Membranes, PLoS One, 5, e9830.

N.C. Santos, J.M. Silva, T. Freitas, M. Doroana, N. Duarte, L. Tavares, F. Antunes, C. Saldanha (2010) Blood cell membrane fluidity and intracellular Ca2+ changes in antiretroviral-naïve and -treated HIV-1-infected patients, ScientificWorldJournal, 10, 350-355.

H.G. Franquelim, A.S. Veiga, G. Weissmüller, N.C. Santos, M.A. Castanho (2010) Unravelling the molecular basis of the selectivity of the HIV-1 fusion inhibitor sifuvirtide towards phosphatidylcholine-rich rigid membranes, Biochim. Biophys. Acta, 1798, 1234-1243.

 

2009

M.M. Domingues, M.A.R.B. Castanho, N.C. Santos (2009) rBPI(21) promotes lipopolysaccharide aggregation and exerts its antimicrobial effects by (hemi)fusion of PG-containing membranes, PLoS One, 4, e8385.

M.M. Domingues, S.C. Lopes, N.C. Santos, A. Quintas, M.A. Castanho (2009) Fold-unfold transitions in the selectivity and mechanism of action of the N-terminal fragment of the bactericidal/permeability-increasing protein (rBPI(21)), Biophys. J., 96, 987-996.

 

2008

H.G. Franquelim, L.M.S. Loura, N.C. Santos, M.A.R.B. Castanho (2008) Sifuvirtide screens rigid membrane surfaces. Establishment of a correlation between efficacy and membrane domain selectivity among HIV fusion inhibitor peptides, J. Am. Chem. Soc., 130, 6215.

P.S. Santiago, F. Moura, L.M. Moreira, M.M. Domingues, N.C. Santos, M. Tabak (2008) Dynamic light scattering and optical absorption spectroscopy study of pH and temperature stabilities of the extracellular hemoglobin of Glossoscolex paulistus, Biophys. J., 94, 2228-2240.

P.M. Matos, S. Gonçalves, N.C. Santos (2008) Interaction of peptides with biomembranes assessed by potential sensitive fluorescent probes, J. Pept. Sci., 14, 407-415.

C.R. Vieira, M. Castanho, C. Saldanha, N.C. Santos (2008) Enfuvirtide effects on human erythrocytes and lymphocytes functional properties, J. Pept. Sci., 14, 448-454.

M.M. Domingues, P.S. Santiago, M.A.R.B. Castanho, N.C. Santos (2008) What can light scattering spectroscopy do for membrane-active peptide studies?, J. Pept. Sci., 14, 394-400.

F. Morinha,  P. Travassos, F. Seixas, A. Martins, R. Bastos, D. Carvalho, P. Magalhães, M. Santos, E. Bastos, J.A. Cabral (2014) Differential mortality of birds killed at wind farms in Northern Portugal. Bird Study, 61, 255-259. (DOI: 10.1080/00063657.2014.883357)

 



 

Av. Professor Egas Moniz
Edifício Egas Moniz,
1649-028 Lisboa
Portugal
 

Tel: (+351)217985136
Fax: (+351)217999477


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